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trifuged at 1000 g for 10 minutes at +4°C. The supernatant was collected and ... was added to the incubation solution, giving a final concentration of 0.08 nM for 2 hr/1 hr at ... binding program EBDA/LIGAND (21) or Multicalc Software (LKB Wallac, .... hCGRP and CGRP Analogs TV5491, TV5591, TV5691, TV5791, TV6191.