W W W .K R O M A T O N .C O M

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3 grammes of this extract which contains 0.3% in weight of hypericin is injected. Detection is done with ELSD detector PL-ELS 2100 from Polymer laboratories.
FCPC® APPLICATION NOTE N°04005

General fractionnation of Hypericum perforatum extract on Bench scale FCPC® equipped with 1L rotor Kromaton application laboratory, Angers, France

Introduction Hypericum perforatum L. is a herbaceous perennial plant, belonging to Hypericacea family. It is a well know medicinal plant since the antiquity and was recognized as “St. John’s Wort” in Anglo-Saxon folk medicine. The extract of aerial part in blossom had a high reputation as an anti-inflammatory, anti depressive and healing agent. Nowadays the use of Hypericum extracts is concerned mainly with antidepressive applications. ® Bench scale FCPC equipped with 1L rotor is used to fractionated commercial extract of Hypericum perforatum in order to isolate active compound as hypericin, pseudo hypericin, hyperforin. 3 grammes of this extract which contains 0.3% in weight of hypericin is injected. Detection is done with ELSD detector PL-ELS 2100 from Polymer laboratories.

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Results

ELSD Chromatogram of FCPC fractionation

Parameters Flow-rate Rotation speed Solvent system Quantity injected

20 ml/mn 1000 rpm Hept/AcOEt/MeOH/W 3 gr

Results Separation time Solvent consumption

115 mn 2,3 L

Conclusions Light Scattering detector allows high detection level on preparative CPC as baseline is sometimes noisy with UV due to cloudy mobile phase or emulsion. In this case, the fractionation is focused on hypericin and pseudo hypericin compound, and solvent system is chosen according to their polarity. Moreover, the dual mode, which allows working in normal and reversed mode in one step, permit to recover the integrity of the crude complex extract from high to low polar compounds.

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